DHEA诱导雌性大鼠PCOS模型早期性器官及其相关激素变化的研究

doi:10.11843/j.issn.0366-6964.2015.08.010

摘要/Abstract

摘要：

本研究旨在探讨大鼠卵巢囊肿形成过程中，大鼠性腺等器官增重、性激素水平以及卵巢组织抗苗勒管激素AMH mRNA变化关系。选取性未成熟的雌性SD大鼠80只(21 d)，随机分为两组：试验组和对照组各40只。试验组大鼠颈部皮下注射0.2 mL•d^-1芝麻油+DHEA（每100 g体重注射6 mg DHEA）；对照组注射等量注射用油，预试期2 d，正试期20 d。分别在试验开始第5、10、15和20天称取大鼠的体重、卵巢、子宫、肝、肾和脾的重量，计算脏器指数，采集血液备用。结果显示，第15和20天试验组大鼠的体重高于对照组(P<0.01)；试验组大鼠子宫重量显著高于对照组(P<0.01)，第5和10 天卵巢重量显著高于对照组(P<0.01)；试验组血清AMH和雄激素（T）均显著高于对照组(P<0.01或P<0.05)，第15和20 天血清P以及第20天血清LH和E₂试验组均显著高于对照组(P<0.01或P<0.05)；试验组卵巢AMH mRNA表达量高于对照组，第5和10天表达量差异显著(P<0.05)。结果提示，试验组大鼠性器官在性发育早期得到优先发育；抗苗勒管激素异常分泌可能是大鼠PCOS发病机理和发展的标志；卵巢AMH mRNA表达与血清AMH分泌变化规律并不完全一致，可能为深入研究多囊卵巢综合征的发生机制提供依据。

Abstract:

This experiment was designed to study the effects of dehydroeplandrosterone (DHEA) on reproductive organs development，hormones level in serum and AMH mRNA expression with the development of rat polycystic ovarian syndrome (PCOS).Eighty immature female SD rats(21 d) were randomly divided into 2 groups：control group with no DHEA(n=40) and treatment group with DHEA(6 mg•（100 g body weight）^-1，n=40).Rats were injected daily with 0.2 mL seame oil for up to 20 days.Ten rats of each groups were killed at 5，10，15 and 20 days.The blood samples were obtained，and organ indexes were calculated by weights of body，ovary，uterus，livers，kidney，and spleen.The detection results showed that the weights of body (15，20 days)，uterus (5，10，15，20 days) and ovarian (5，10 days)of treatment groups were significantly higher than the control groups (P<0.01)；Serum AMH and T of treatment groups (5，10，15，20 days) were significantly higher than control groups(P<0.01 or P<0.05)，while the serum P (15，20 days)，LH and E₂ (20 days) were higher (P<0.01 or P<0.05)；The levels of AMH mRNA of treatment groups(5，10 days) were obvious different significantly higher (P<0.05).These results indicated that the sexual organs were given priority to development during early stage of prepuberty in treated group.The abnormal AMH production could be cause of pathogenesis and development marker of PCOS in rats.The expression of AMH mRNA in ovary and serum AMH level were not exactly the same，the results may supply the reference data for further research on PCOS in female rats.

中图分类号:

S852.23

郭保平，徐明龙，胡进，徐银学. DHEA诱导雌性大鼠PCOS模型早期性器官及其相关激素变化的研究[J]. 畜牧兽医学报, 2015, 46(8): 1348-1355.

GUO Bao-ping，XU Ming-long，HU Jin，XU Yin-xue. Studies on Changes of Sexual Organs and Their Related Hormones during the Early Phase in Dehydroepiandrosterone-induced Female Rat Polycystic Ovarian Syndrome Model[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1348-1355.

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https://www.xmsyxb.com/CN/Y2015/V46/I8/1348

参考文献

［1］RUTKOWSKA A，RACHON D.Bisphenol A (BPA) and its potential role in the pathogenesis of the polycystic ovary syndrome (PCOS)［J］.Gynecol Endocrinol，2014，30(4)：260-265.
［2］ERIKSEN M B，NIELSEN M F，BRUSGAARD K，et al.Genetic alterations within the DENND1A gene in patients with polycystic ovary syndrome (PCOS)［J］.PLoS ONE，2013，8(9)：e77186.
［3］DIAMANTI-KANDARAKIS E，CHRISTAKOU C，MARINAKIS E.Phenotypes and enviromental factors：their influence in PCOS［J］.Curr Pharm Des，2012，18(3)：270-282.
［4］KEDEM A，HOURVITZ A，YUNG Y，et al.Anti-Mullerian hormone (AMH) downregulation in late antral stages is impaired in PCOS patients.A study in normo-ovulatory and PCOS patients undergoing in vitro maturation (IVM) treatments［J］.Gynecol Endocrinol，2013，29(7)：651-656.
［5］KOTANIDIS L，ASIMAKOPOULOS B，NIKOLETTOS N.Association between AMH，oocyte number and availability of embryos for cryopreservation in IVF［J］.In vivo，2013，27(6)：877-880.
［6］PEIGNE M，DECANTER C.Serum AMH level as a marker of acute and long-term effects of chemotherapy on the ovarian follicular content：a systematic review［J］.Reprod Biol Endocrinol，2014，12：26.
［7］LEE M T，ANDERSON E，LEE G Y.Changes in ovarian morphology and serum hormones in the rat after treatment with dehydroepiandrosterone［J］.Anat Rec，1991，231(2)：185-192.
［8］KONG J S，YOO S A，KIM J W，et al.Anti-neuropilin-1 peptide inhibition of synoviocyte survival，angiogenesis，and experimental arthritis［J］.Arthritis Rheum，2010，62(1)：179-190.
［9］BRAWER J R，MUNOZ M，FAROOKHI R.Development of the polycystic ovarian condition (PCO) in the estradiol valerate-treated rat［J］. Biol Reprod，1986，35(3)：647-655.
［10］HOMBURG R，CRAWFORD G.The role of AMH in anovulation associated with PCOS：a hypothesis［J］.Hum Reprod，2014，29(6)：1117-1121.
［11］周宁，张海华，吕智超，等.饲粮锌水平对冬毛期水貂血清生化指标和脏器指数的影响［J］.畜牧兽医学报，2014，45（12）：1988-1994.
ZHOU N，ZHANG H H，LÜ Z C，et al.Effects of diets with different zinc levels on the selected serum biochemical indexes and organ indexes of minks during the winter hair period［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(12)：1988-1994.（in Chinese）
［12］ANDERSON A D，SOLORZANO C M，MCCARTNEY C R.Childhood obesity and its impact on the development of adolescent PCOS［J］.Semin Reprod Med，2014，32(3)：202-213.
［13］王波，姜成钢，纪守坤，等.日粮尿素水平对肉羊血液学、血清学指标和消化器官的影响［J］.畜牧兽医学报，2014，45（9）：1449-1456.
WANG B，JIANG C G，JI S K，et al.Effect of different levels of urea in diets on haematological indices，serological parameters and tissue lesions in Mutton sheep［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(9)：1449-1456.（in Chinese）
［14］韩芸，参芪胶囊改善PCOS子宫内膜容受性的临床与试验研究［D］.广州：广州中医药大学，2013.
HAN Y.The clinical and experimental study on improvement endometrial receptivity effects of Shen-Qi capsule in polycystic ovary syndrome［D］.Guangzhou：Guangzhou University of Chinese Medicine，2013.（in Chinese）
［15］SHAFIEE M N，KHAN G，ARIFFIN R，et al.Preventing endometrial cancer risk in polycystic ovarian syndrome (PCOS) women：could metformin help?［J］.Gynecol Oncol，2014，132(1)：248-253.
［16］GIUDICE L C.Endometrium in PCOS：Implantation and predisposition to endocrine CA［J］.Best Pract Res Clin Endocrinol Metab，2006，20(2)：235-244.
［17］张宝乐.猪GPR3基因特征、表达及其对卵泡颗粒细胞的影响［D］.南京：南京农业大学，2012.
ZHANG B L.Characterization，expression of porcine GPR3 gene and its effects on porcine follicular granulosa cells［D］.Nanjing： Nanjing Agricultural University，2012.（in Chinese）
［18］汤娟.多囊卵巢综合征（PCOS）的诊断及中医对其病理生理认识的研究［D］.长沙：湖南中医药大学，2014.
TANG J.Study on the clinical diagnosis of PCOS and the understanding of the pathophysiology of its in TCM［D］.Changsha：Hunan University of Chinese Medicine，2014.（in Chinese）
［19］TAYLOR A E，MCCOURT B，MARTIN K A，et al.Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome［J］.J Clin Endocrinol Metab，1997，82(7)：2248-2256.
［20］BATTAGLIA C，NAPPI R E，MANCINI F，et al.PCOS and urethrovaginal space：3-D volumetric and vascular analysis［J］.J Sex Med，2010，7(8)：2755-2764.
［21］沈宗奇.多囊卵巢综合征卵泡发育异常的相关因素及机制研究［D］.上海：复旦大学，2009.
SHEN Z Q.Correlation factors and mechanism involved in the developmental anomaly of follieles in polyeystic ovary syndrome［D］.Shanghai：Fudan University，2009.（in Chinese）
［22］郭芳，罗国静，鞠志花，等.SPEF2基因可变剪切体和功能性SNP鉴定及其与公牛精液性状的相关性研究［J］.南京农业大学学报，2014，37(3)：119-125.
GUO F，LUO G J，JU Z H，et al.Association of SPEF2 gene splices variant and functional SNP with semen quality traits in Chinese Holstein bulls［J］.Journal of Nanjing Agricultural University，2014，37(3)：119-125.（in Chinese）
［23］IMHOFF F M，YANG D，MATHEW S F，et al.The type 2 anti-Müllerian hormone receptor has splice variants that are dominant-negative inhibitors［J］.FEBS Lett，2013，587(12)：1749-1753.
［24］徐燕颖.抗苗勒氏管激素在多囊卵巢综合征中的表达及相关机制研究［D］.天津：天津医科大学，2013.
XU Y Y，The expression and related mechanism of anti-Müllerian hormone in polycystic ovary syndrome［D］.Tianjin：Tianjin Medical University，2013.（in Chinese）
［25］XU P，SHEN S M，ZHANG X L，et al.Haplotype analysis of single nucleotide polymorphisms in anti-Müllerian hormone gene in Chinese PCOS women［J］.Arch Gynecol Obstet，2013，288(1)：125-130.
［26］ESSAH P A，NESTLER J E，CARMINA E.Differences in dyslipidemia between American and Italian women with polycystic ovary syndrome［J］.J Endocrinol Invest，2008，31(1)：35-41.
［27］YU N E，HO P C.Polycystic ovary syndrome in Asian women［J］.Semin Reprod Med，2008，26(1)：14-21.
［28］SHI Y，GUO M，YAN J，et al.Analysis of clinical characteristics in large-scale Chinese women with polycystic ovary syndrome［J］.Neuro Endocrinol Lett，2007，28(6)：807-810.

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